Thermo Fisher Scientific Phospho-ERK1/2 (Thr202, Tyr204) Monoclonal Antibody (MILAN8R), eBioscience

Thermo Fisher Scientific Phospho-ERK1/2 (Thr202, Tyr204) Monoclonal Antibody (MILAN8R), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• APC
• PE
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This MILAN8R monoclonal antibody recognizes human and mouse extracellular signal-regulated kinases 1 and 2 (ERK1/2, also known as p44/p42 or MAPK3/1) when phosphorylated on T202/Y204. ERK1/2 are conserved serine/threonine kinases involved in cellular processes such as proliferation, differentiation, motility, and survival. Their activation occurs via MEK1 and MEK2 phosphorylation, and they regulate downstream targets including p90RSK and Elk-1. ERK pathway dysregulation is common in various cancers.

Applications Reported:
Suitable for intracellular staining (Flow cytometry), Western blotting, immunohistochemistry (FFPE), and immunocytochemistry. Fluorochrome-conjugated MILAN8R is recommended for intracellular flow cytometry.

Applications Tested:
Validated by immunoblotting of stimulated Jurkat cells (≤5 µg/mL), immunohistochemistry on FFPE human tissue (low pH antigen retrieval), and immunocytochemistry of MeOH-fixed cells (≤5 µg/mL). Optimal dilution should be determined experimentally.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

ERK1 and ERK2 are key regulators of meiosis, mitosis, and postmitotic functions in differentiated cells. They are activated by various stimuli such as growth factors, cytokines, and viral infections. Activation occurs through the Ras-Raf-MEK-ERK cascade, leading to phosphorylation of cytoplasmic and nuclear targets, including transcription factors c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.