Thermo Fisher Scientific HSP70 Monoclonal Antibody (1B2hsp70), eBioscience
HSP70 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC에 사용 가능. 개·인간·비인간 영장류 반응성. 액상 형태, 0.5 mg/mL, 4°C 보관. 열충격 단백질 연구 및 스트레스 반응 관련 연구에 적합.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific HSP70 Monoclonal Antibody (1B2hsp70), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Non-human primate |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B2hsp70 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865351 |
Additional Formats
Product Specific Information
Description:
The monoclonal antibody 1B2hsp70 recognizes HSP70 (heat shock protein 70). HSPs are highly conserved molecular chaperones that stabilize or assist in folding of new or denatured proteins following stress. Expression of HSPs can be constitutive or inducible depending on cell type and environmental conditions. Upregulation of HSPs is observed in cancer, ischemic injury, inflammatory, and demyelinating diseases.
This antibody recognizes HSP70 family member 1A located in the cytoplasm and nucleus of many cell types. Due to high conservation, clone 1B2hsp70 is expected to recognize canine and monkey HSP70 1A.
Applications Reported:
- Western blotting
- Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
- Microscopy
Applications Tested:
Tested by IHC on FFPE tissue using both low and high pH antigen retrieval at ≤5 µg/mL. Recommended to titrate for optimal performance.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
HSP70 is a heat shock protein expressed in response to various stresses such as elevated temperature. Major HSP families include HSP70, HSP90, and HSP100. The HSP70 family consists of highly conserved proteins induced by diverse stresses in all studied organisms.
Human HSP70 family members include:
- HSP70: Strongly inducible but also constitutively expressed in primate cells
- HSP72: Induced exclusively under stress conditions
- HSC70: Constitutively expressed, involved in clathrin-coated vesicle uncoating
- GRP78 (BiP): ER-localized, glucose-regulated 78 kDa protein
- p75 (HSP75): Mitochondrial protein
HSP70 is encoded by an intronless gene within the MHC class III region, clustered with two related genes. It stabilizes proteins against aggregation, assists folding of new proteins, and participates in the ubiquitin-proteasome pathway via interaction with AU-rich element RNA-binding protein 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.