Thermo Fisher Scientific HSP27 Monoclonal Antibody (STRSN), eBioscience
Human HSP27 단백질을 인식하는 STRSN 클론의 Mouse Monoclonal Antibody로, Western blot, IHC, ICC, ELISA에 사용 가능. 스트레스 반응 및 세포 사멸 조절 연구에 적합하며, 높은 순도(>90%)와 안정적인 액상 형태로 제공.
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Thermo Fisher Scientific HSP27 Monoclonal Antibody (STRSN), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1–2.5 µg/mL | - |
| ELISA | Assay-Dependent | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | STRSN |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219272 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The monoclonal antibody STRSN recognizes human heat shock protein 27 (HSP27). Heat shock proteins are induced in response to stressors such as temperature changes, cytokine levels, and various chemicals. They act as molecular chaperones, maintaining protein structure and preventing apoptosis by inhibiting caspase activation.
HSP27 is involved in apoptosis regulation and relocates from the cytoplasm to the nucleus upon stress. Elevated HSP27 levels are associated with aggressive tumors and reduced survival in breast cancer and melanoma. Increased serum HSP27 may serve as a diagnostic marker in COPD, breast cancer, and melanoma. No cross-reactivity observed with HSP70, HSP90, HSP104, or HSP110.
Applications Reported:
Western blotting, immunohistochemistry (FFPE tissue), ELISA, and immunocytochemistry.
Applications Tested:
Validated on reduced HeLa lysate (WB), fixed A549 cells (ICC), and FFPE human tissue (IHC) at ≤1 µg/mL. Optimal titration recommended.
Target Information
HSPB1 (HSP27) is a small heat shock protein functioning as a molecular chaperone that maintains denatured proteins in a folding-competent state. It contributes to stress resistance and actin organization, regulating phosphorylation and axonal transport of neurofilament proteins. Mutations in HSPB1 may cause Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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