Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD85d (ILT4) Monoclonal Antibody (42D1), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 2 publications

Product Specifications

Available Formats

• APC
• Functional Grade
• NovaFluor Red 710
• PE
• PE-Cyanine7
• Custom conjugation available

Product Specific Information

The monoclonal antibody 42D1 recognizes CD85d (ILT4, LIR-2), a 110 kDa single transmembrane receptor with three Ig-like domains and a long cytoplasmic tail containing ITIMs.
Expression is confined to monocytes, macrophages, and dendritic cells. The ligand for CD85d includes non-classical class I HLA-G, -B, and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM, contributing to immune tolerance during fetal implantation and playing a role in organ allograft rejection.

Crosslinking with the 42D1 antibody inhibits calcium flux, enhances HLA-G tetramer staining, and restores the stimulatory activity of tolerogenic dendritic cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Fluorochrome Information:
PerCP-eFluor™ 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Excitation: 488 nm
• Emission: 710 nm
• Laser: Blue Laser
  Recommended filters: 685 LP dichroic mirror and 710/40 band pass (or 695/40 alternative).

PerCP-eFluor™ 710 conjugated antibodies are stable after overnight exposure to 2% formaldehyde at 4°C. Evaluate alternative fixation protocols as needed.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Gene: LILRB2 (ILT4)
Located on chromosome 19q13.4, part of the leukocyte immunoglobulin-like receptor (LIR) family.
Encodes a receptor expressed on immune cells that binds MHC class I molecules on antigen-presenting cells, transmitting inhibitory signals to modulate immune responses.
Helps control inflammation and cytotoxicity, limiting autoreactivity. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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