Thermo Fisher Scientific c-Met Monoclonal Antibody (A9A5)
인간 c-Met 단백질을 인식하는 Mouse IgG1 단클론 항체. Western blot 및 IHC(Paraffin)에서 검증됨. 단백질 A로 정제된 액상 항체로, 1 mg/mL 농도. c-Met 관련 암 연구 및 신호전달 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A9A5 |
| Immunogen | Synthetic peptide within Human c-Met aa 1341–1390/1390 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3091233 |
Product Specific Information
- Positive control: A549 cell lysate, HepG2 cell lysate, human lung carcinoma tissue
- Predicted band size: 156 kDa
- Subcellular Location: Membrane; Secreted
Target Information
MET (c-Met) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. These interactions lead to activation of signaling cascades including RAS-ERK, PI3K-AKT, and PLCγ-PKC pathways.
MET is involved in embryonic development processes such as gastrulation, muscle and neuron development, angiogenesis, and kidney formation. In adults, it contributes to wound healing, organ regeneration, and tissue remodeling.
Abnormal MET signaling has been associated with cancers including gastric, renal, and breast cancers, making it a key target for cancer therapeutics and diagnostic research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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