Thermo Fisher Scientific AP2 alpha Monoclonal Antibody (3B5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50 - 1:200

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

1:100 - 1:250

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

N-terminus of human AP2a. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AP2 alpha,uniProtId:P05549-1,ncbiNodeId:9606,antigenRange:1,antigenLength:437,antigenImageFileName:MA1-872_AP2_alpha_P05549-1_House_mouse.svg,antigenImageFileNamePDP:MA1-872_AP2_alpha_P05549-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2199412

Product Specific Information

MA1-872 detects AP-2 from human, mouse, and chicken samples.

MA1-872 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence applications. By Western blot, this antibody detects a 45-50 kDa band representing AP-2. For Western blotting, block with BSA only (not milk).

The MA1-872 immunogen is the N-terminus of human AP2a.

Target Information

TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5-GCCNNNGGC-3 and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.