Thermo Fisher Scientific HEF1 Monoclonal Antibody (14A11)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

5 µg/mg whole cell lysate

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

14A11

Immunogen

GST fusion protein, corresponding to amino acids 82-398 of Human HEF1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HEF1,uniProtId:Q14511-1,ncbiNodeId:9606,antigenRange:82-398,antigenLength:834,antigenImageFileName:MA1-90001_HEF1_Q14511-1_House_mouse.svg,antigenImageFileNamePDP:MA1-90001_HEF1_Q14511-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2149480

Product Specific Information

MA1-90001 detects HEF1 from human samples.

Target Information

HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.