Thermo Fisher Scientific Phospho-L-Plastin (Tyr300) Polyclonal Antibody
인간 및 마우스 시료에서 Tyr300 인산화된 L-Plastin을 검출하는 폴리클로날 항체. Western blot 및 면역세포염색에 적합. 고순도 정제 및 1 mg/mL 농도의 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human LCP1 (Accession P13796), corresponding to amino acid residues around phosphorylated Tyr300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816921 |
Product Specific Information
Antibody detects endogenous levels of Plastin L only when phosphorylated at Tyr300.
Target Information
L-plastin is a leukocyte-specific actin-bundling protein from the fimbrin family. It contains two actin-binding domains and two EF-hand type calcium-binding domains. Phosphorylation occurs on the serine residue in its headpiece region in response to inflammatory stimuli. It is hypothesized that L-plastin plays a role in regulating integrin-mediated adhesion in leukocytes. L-plastin is expressed in most human cancer cell lines derived from solid tumors and is upregulated by testosterone in androgen receptor-positive prostate and breast cancer cells through three androgen receptor binding elements in the gene’s upstream region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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