Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific HLA-ABC Monoclonal Antibody (W6/32), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 395
• Brilliant UV 615
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• Functional Grade
• NovaFluor Blue / Red / Yellow series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436, 600, 702
• Custom conjugation available

Product Specific Information

The W6/32 monoclonal antibody reacts with human MHC class I antigens (HLA-A, B, C) associated with beta-2 microglobulin. These antigens are expressed on all nucleated cells and are key in cell-mediated immune response and tumor surveillance.
W6/32 recognizes a non-polymorphic epitope shared among HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11–12 kDa chains. Cross-reactivity is observed in baboon, rhesus, and cynomolgus monkey.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted; use 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for cytometers equipped with a 355 nm ultraviolet laser. Ensure compatibility with your instrument.

Staining Buffer Recommendations

When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401)
• Brilliant Stain Buffer (Product # 00-4409)

These buffers minimize non-specific polymer interactions.

Light Sensitivity

This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation Compatibility

Samples may be stored in:

• IC Fixation Buffer (Product # 00-8222)
• 1-step Fix/Lyse Solution (Product # 00-5333)

Storage up to 3 days at 4°C in the dark is possible with minimal impact on brightness.
BUV737 is compatible with short-term methanol fixation (<1 hour).

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

HLA-class I MHC antigens are membrane glycoproteins expressed on nucleated cells, associated with beta2 microglobulin. They present foreign determinants for recognition by cytotoxic T cells, crucial for antiviral and antitumor defense.
HLA and MHC antibodies are key in immunopeptidomics, aiding identification of neoantigens via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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