Thermo Fisher Scientific ErbB4 Monoclonal Antibody (H4.77.16 (Ab77))

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

- -

Immunocytochemistry (ICC/IF)

2 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/test

View 4 publications 4 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mg protein lysate

View 2 publications 2 publications

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

H4.77.16 (Ab77)

Immunogen

Extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986112

Product Specific Information

MA5-12888 targets HER-4 in Western blot, flow cytometry and immunoprecipitation applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-12888 immunogen is extracellular fragment of recombinant human c-erbB-4/HER-4 oncoprotein.

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.