Thermo Fisher Scientific EGFR Monoclonal Antibody (225)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 10 publications 10 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

Immunoprecipitation (IP)

2 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

-

View 9 publications 9 publications

Gel Shift (GS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

225

Immunogen

Purified EGFR from A431 cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EGFR,uniProtId:P00533-1,ncbiNodeId:9606,antigenRange:1-1210,antigenLength:1210,antigenImageFileName:MA5-12880_EGFR_P00533-1_House_mouse.svg,antigenImageFileNamePDP:MA5-12880_EGFR_P00533-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10982150

Product Specific Information

MA5-12880 targets Epidermal Growth Factor Receptor in IP and IF applications and shows reactivity with Human and Non-human primate samples.

The MA5-12880 immunogen is purified EGFR from A431 cells.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.