Thermo Fisher Scientific MYOD Monoclonal Antibody (5.2F)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

2 µg/mL

-

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

5.2F

Immunogen

Recombinant mouse MyoD1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979741

Product Specific Information

MA5-12907 targets MyoD1 in IF, IM, and IP applications and shows reactivity with Chicken, Human, mouse, and Rat samples.

The MA5-12907 immunogen is recombinant mouse MyoD1 protein.

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.