Thermo Fisher Scientific VISTA Monoclonal Antibody (CL3981)

Applications

Immunohistochemistry (Paraffin) (IHC-P)

• Tested Dilution: 1:1,000–1:2,000

Product Specifications

Product Specific Information

Immunogen sequence:
LTFQDLHLHHG GHQAANTSHD LAQRHGLESA SDHHGNFSIT MRNLTLLDSG LYCCLVVEIR HHHSEHRVHG AMELQVQTGK DAPSNCVVYP SSS

• Binds to an epitope located within the peptide sequence LHLHHGGHQA as determined by overlapping synthetic peptides.
• Highest antigen sequence identity to the following orthologs: mouse 60%, rat 59%.

Target Information

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene.
Although VISTA bears sequence homology to PD-L1, it has a distinct expression pattern.
In both mouse and humans, VISTA is predominantly expressed on hematopoietic cells, with the highest densities on myeloid cells and lower levels on T cells, and is typically absent on B cells.
VISTA expressed by antigen-presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy.
Reported ligands include CD28H, VSIG-3, and VSIG-8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.