Thermo Fisher Scientific Dynein Monoclonal Antibody (74.1)
Western blot, ICC/IF, Flow cytometry, IP, RIP 등 다양한 응용에 사용 가능한 Dynein 단클론 항체. Bovine, Human, Mouse, Rat 반응성. 단백질 A 정제, 액상형, 농도 1 mg/mL. 세포 내 세포골격 수송 단백질 Dynein 검출용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 4 publications |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | 1–2 µg/test | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | View 2 publications |
| RNA Immunoprecipitation (RIP) | Assay-dependent | — |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Bovine, Human, Many, Mouse, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 74.1 |
| Immunogen | Purified bovine brain cytoplasmic dynein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2093668 |
Product Specific Information
MA1-070 detects two isoforms of the ~74 kDa polypeptide of cytoplasmic dynein.
It has been successfully used in Western blot, immunofluorescence, and immunoprecipitation assays.
In Western blot analysis of HeLa cell lysate, this antibody detects a ~74 kDa protein representing cytoplasmic dynein.
The immunogen used is purified bovine brain cytoplasmic dynein.
Target Information
The directed movement of membrane-bounded organelles is essential for cellular functions such as membrane transport, secretion, and axonal transport.
Cytoplasmic dynein is a ubiquitous, minus end-directed, microtubule-based motor protein responsible for retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport.
The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains and intermediate chains of ~74 kDa and ~53–59 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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