Thermo Fisher Scientific Dynein Monoclonal Antibody (74.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

2-4 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

1-2 µg/test

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

RNA Immunoprecipitation (RIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Many, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

74.1

Immunogen

Purified bovine brain cytoplasmic dynein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Dynein,uniProtId:P61285-1,ncbiNodeId:9913,antigenRange:1-89,antigenLength:89,antigenImageFileName:MA1-070_Dynein_P61285-1_House_mouse.svg,antigenImageFileNamePDP:MA1-070_Dynein_P61285-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2093668

Product Specific Information

MA1-070 detects two isoforms of the ~74 kDa polypetide of cytoplasmic dynein.

MA1-070 has been successfully used in Western blot immunofluorescence, and immunoprecipitation procedures. In Western blot analysis of HeLa cell lysate this antibody detects a ~74 kDa protein representing cytoplasmic dynein.

The MA1-070 immunogen is purified bovine brain cytoplasmic dynein.

Target Information

The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.