Thermo Fisher Scientific HSP90 alpha Monoclonal Antibody (2G5.G3), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2G5.G3

Immunogen

Recombinant Full Length Human HSP90alpha Protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSP90 alpha,uniProtId:P07900-1,ncbiNodeId:9606,antigenRange:1-732,antigenLength:732,antigenImageFileName:MA5-45182_HSP90_alpha_P07900-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45182_HSP90_alpha_P07900-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PerCP PerCP PerCP

View additional formats

• APC
• FITC
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931636

Product Specific Information

0.5 µg/mL of MA5-45182 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 90kDa. HSP90 alpha -specific (>96% alpha -specific by ELISA)

Target Information

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.