Thermo Fisher Scientific E2F4 Monoclonal Antibody (OTI11H1), TrueMAB
E2F4 단백질을 인식하는 마우스 단클론 항체로 Western blot에 최적화되어 있습니다. Human, Mouse, Rat에 반응하며, 무보존제 동결건조 형태로 제공됩니다. 재구성 후 1 mg/mL 농도로 사용 가능하며, 연구용으로 적합합니다.
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Applications
- Western Blot (WB): 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI11H1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 86–413 of human E2F4 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
E2F transcription factors are regulated by binding to Rb p110, p107, and p130.
E2F-4 specifically interacts with Rb p110 and binds DNA as a heterodimer with DP family proteins.
Differential phosphorylation contributes to the microheterogeneity of E2F-4 (total aa 411–416, varies due to a trinucleotide repeat).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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