Thermo Fisher Scientific RLIM Monoclonal Antibody (OTI2D7), TrueMAB
RLIM 단백질을 표적하는 마우스 단클론 항체로, 인간·마우스·랫트 반응성을 보임. Western blot과 IHC(P) 분석에 적합하며, 고순도의 친화 크로마토그래피 정제 제품. PBS/trehalose 완충액에 동결건조 형태로 제공되어 안정적 보관 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2D7 |
| Immunogen | Full length human recombinant protein of human RLIM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
RNF12 is a RING-H2 zinc finger protein functioning as a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), leading to proteasome-dependent degradation of LDB1.
RNF12 and LDB1 act as co-repressors of LHX1/LIM-1, a homeodomain transcription factor.
Alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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