Thermo Fisher Scientific CDK7 Recombinant Rabbit Monoclonal Antibody (BL-80-5D4)
CDK7 단백질을 인식하는 Rabbit 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. HEK293 세포에서 발현된 재조합 항체이며, 고순도의 액상 형태로 제공. 인간 CDK7 단백질 C-말단 부위(300–346 aa)를 면역원으로 제작됨. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:25–1:100 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1 µL per 1 × 10⁶ cells |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BL-80-5D4 |
| Immunogen | Synthetic peptide representing a region between residues 300 and the C-terminus (residue 346) of human Cyclin-Dependent Kinase 7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 40 µg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- Application Note:
- For ICC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The trimeric complex of CDK7, cyclin H, and MNAT1 functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, which is involved in transcription initiation and DNA repair. This complex serves as a direct link between transcription regulation and the cell cycle. The bound complex is important in the normal double-strand break repair function of BRCA1. CDK7 may be a target of germline cancer-inducing mutations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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