Thermo Fisher Scientific CDK7 Recombinant Rabbit Monoclonal Antibody (BL-80-5D4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (IHC)

1:25-1:100

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

1 µL per 1 X 10^6 cells

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

BL-80-5D4

Immunogen

Synthetic peptide representing a region between residues 300 and the C-terminus (residue 346) of human Cyclin-Dependent Kinase 7. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CDK7,uniProtId:P50613-1,ncbiNodeId:9606,antigenRange:300-346,antigenLength:346,antigenImageFileName:A700-006_CDK7_P50613-1_Rabbit.svg,antigenImageFileNamePDP:A700-006_CDK7_P50613-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

40 µg/mL

Purification

Ion-exchange chromatography

Storage buffer

BBS, pH 8.2, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For ICC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

The trimeric complex of CDK7, cyclin H and MNAT1, functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This complex is thought to serve as a direct link between the regulation of transcription and the cell cycle. The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). CDK7 may be a target of germline cancer-inducing mutations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.