Thermo Fisher Scientific beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight 550
DyLight 550 형광 표지를 가진 베타 튜불린 단클론 항체로, 로딩 컨트롤 및 면역형광(ICC/IF)에 적합. 마우스 IgG2a 모노클론 항체이며, 556/570 nm 형광 특성. 신경세포 및 일반 세포의 튜불린 검출에 활용 가능.
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Thermo Fisher Scientific beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight 550
Applications
- Immunocytochemistry (ICC/IF)
Tested Dilution: 1:25–1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BT7R |
| Immunogen | KLH conjugated peptide of the N-terminus of Beta-Tubulin |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2537823 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Biotin
- DyLight 488
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- HRP
- Request custom conjugation available
Product Specific Information
MA5-16308-D550 has successfully been used for immunofluorescence.
DyLight 550 has an excitation/emission of 562/576 nm.
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Glutamate residues at the C-terminus of beta III tubulin can be glutamylated, though the precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, inhibiting tubulin incorporation into microtubules.
Microtubules, major cytoskeletal elements in all eukaryotic cells, consist of Tubulin dimers (alpha and beta, ~55 kDa each). They play key roles in chromosome segregation, intracellular transport, ciliary and flagellar movement, and structural support.
Because beta-tubulin is ubiquitously expressed, it is frequently used as a loading control for assays such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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