Thermo Fisher Scientific beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight 488
DyLight 488으로 표지된 beta Tubulin 단클론 항체로 면역형광(ICC/IF)에 적합. 마우스 IgG2a 아이소타입이며, 세포 내 마이크로튜뷸 구조 확인용 로딩 컨트롤로 활용. 액상 형태로 4°C 보관, 농도 1 mg/mL.
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Applications and Tested Dilution
- Immunocytochemistry (ICC/IF): 1:25–1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BT7R |
| Immunogen | KLH-conjugated peptide of the N-terminus of Beta-Tubulin |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537822 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 647
- Biotin
- DyLight 680
- DyLight 800 4X PEG
- HRP
- Custom conjugation available on request
Product Specific Information
MA5-16308-D488 has been validated for immunofluorescence applications.
DyLight 488 fluorophore exhibits excitation/emission maxima at 493/518 nm.
Target Information
Beta tubulins are one of two core tubulin protein families (alpha and beta) that form microtubules, essential components of the eukaryotic cytoskeleton.
Beta-III tubulin is primarily expressed in neurons and is involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene can cause congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also transiently expressed in non-neuronal embryonic tissues and Sertoli cells.
Tubulin undergoes post-translational modifications such as glutamylation and phosphorylation (e.g., Ser-172 by CDK1), which influence its incorporation into microtubules.
Due to its ubiquitous expression, beta-tubulin is widely used as a loading control in protein detection assays such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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