Thermo Fisher Scientific GRGDS Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated GRGDS.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

GRGDS forms the cell-binding domain of a glycoprotein, Osteopontin (OPN) . Although the native form of OPN is active in cell attachment assays, it has been observed that thrombin cleavage of OPN causes substantial enhancement of it`s attachment properties. GRGDS synthetic peptide also mimics the cellular binding site of many adhesive proteins in the extracellular matrix and causes rounding and detachment of spread cells. It induces dissociation of alpha-actinin and vinculin from the sites of focal contacts. This cleavage occurs within residues of the GRGDS sequence raising the possibility of thrombin-cleavage further activating OPN by allowing greater accessibility of the GRGDS domain to cell surface receptors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.