Norgen Milk DNA Preservation and Isolation Kit

Norgen Milk DNA Preservation and Isolation Kit

SKU: 44800

A rapid all-in-one procedure for the preservation and isolation of milk DNA at ambient temperatures.

Features and Benefits

• Milk samples are stable for 1 month at room temperature (or 1 week at 37°C) in the Preservation Solution
• Fast and easy processing using a rapid spin-column format
• DNA can be isolated and detected from as little as 100 µL of milk
• Isolate high quality genomic DNA

Norgen’s Milk DNA Preservation and Isolation Kit is designed for both preservation of DNA in milk samples at ambient temperature and isolation of high-quality DNA in the lab. The included Preservation Solution allows immediate processing or storage of milk samples for up to 1 month at room temperature before DNA isolation. Extracted genomic DNA from somatic cells and bacteria can be used for biomarker detection, disease diagnosis, and monitoring. The purified DNA is compatible with PCR, Southern blot, sequencing, and microarray analysis.

Milk DNA Preservative

Norgen’s Milk DNA Preservation Solution is an aqueous storage buffer that rapidly lyses cells and preserves DNA from fresh specimens. It prevents microbial growth, inactivates viruses, and allows safe handling and shipping at ambient temperature. Samples can be stored for up to 1 month without detectable DNA degradation.

Supporting Data

Figure 1. Isolation of DNA From Different Volumes of Preserved Milk (0.5 mL, 1 mL, 1.5 mL, and 2 mL)
Ten microlitres of isolated DNA (100 µL elution) were loaded on a 1.2% agarose gel, showing good integrity. DNA yield increased with milk input volume up to 2 mL.

[A] 젤 전기영동 결과
0.5 mL  1 mL  1.5 mL  2 mL  M

[B] DNA 농도 비교 (막대그래프 결과)

Figure 2. Amplification of 16s rRNA Gene From Different Volumes of Preserved Milk
E. coli was spiked into preserved milk, and DNA was isolated using the kit. Two microlitres of DNA were used in a 20 µL qPCR reaction. No PCR inhibition was observed, confirming high DNA quality.

Amplification Curve 정보

• Y축: RFU
• X축: Cycles
• 범례 (Legend):
  • ○— 0.5 mL (분홍색)
  • ○— 1.0 mL (파란색)
  • ○— 1.5 mL (빨간색)
  • ○— 2.0 mL (초록색)

Kit Specifications

Storage Conditions and Product Stability

• Proteinase K should be stored at -20°C upon arrival and after reconstitution.
• All other solutions should be stored tightly sealed at room temperature.
• Kit is stable for 2 years after shipment.

Storage in Preservation Buffer

1. Storage at -20°C is recommended for long-term archival samples. Samples can be stored indefinitely at -70°C.
2. Samples can be stored at room temperature (25°C) for up to 1 month without significant DNA degradation.
3. DNA can also be isolated from samples stored at 37°C for one week.

Kit Components

Documentation

• Protocols: Milk DNA Preservation and Isolation Kit - Protocol (25 prep)
• Product Information Sheet: Milk DNA Preservation and Isolation Kit - Product Information Sheet
• Safety Data Sheet: 44800 - Milk DNA Preservation and Isolation Kit - SDS
• Flyer: Microorganism - Solutions for Isolation and Downstream Applications Flyer

FAQs

Q: The column was clogged. What causes this?

• Centrifugation speed too low or spin time inadequate.
• Sample volume too large (apply ≤1 mL).
• Lysate/Buffer SK mixture not homogeneous (vortex 10–15 sec).

Q: Why is the DNA yield lower than expected?

• Incomplete lysis; increase Proteinase K incubation at 55°C.
• Incomplete elution; ensure centrifugation at 14,000 × g.
• Low DNA content in sample.
• Preservation Solution E not added.

Q: Why is the DNA degraded?

• Preservation Solution E not completely mixed with milk sample.

Q: Why is DNA not performing well in downstream applications?

• Column not washed twice with Wash Solution A.
• Ethanol carryover; ensure dry spin before elution.

Q: Why is RNA present in eluted DNA?

• RNA coeluted with DNA; treat with RNase A if necessary.

Citations

Related Product

Milk Bacterial DNA Isolation Kit (Cat. #21550)
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