Norgen Urine Exosome Purification Mini Kit

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소변 시료에서 엑소좀을 빠르고 간편하게 정제하는 올인원 키트. 초원심분리나 침전 시약 없이 30분 이내 정제 가능. 40–200 nm 크기의 엑소좀을 높은 순도로 확보. 다양한 시료 부피(250 µL–30 mL)에 대응하며 RNA 분석 등 후속 연구에 적합.

카탈로그번호: 57700
브랜드: Norgen
카테고리: Exosome Reagents
판매단위: pk

#R #Norgen #Exosome Reagents #Reagents

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Norgen · Norgen Urine Exosome Purification Mini Kit

Norgen Urine Exosome Purification Mini Kit

SKU: 57700
All-in-one system for the purification of urinary exosomes from different urine sample volumes.

Features and Benefits

Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media, and saliva in less than 30 minutes.
Versatile sample input ranging from 250 µL to 30 mL:
- Mini Kit: 250 µL – 1 mL urine
- Midi Kit: 2 mL – 10 mL urine
- Maxi Kit: 11 mL – 30 mL urine
Based on Norgen’s proprietary resin separating matrix through exosome surface proteins.
No precipitation reagents, overnight incubation, protease, or coagulant treatments required.
No ultracentrifugation, filtration, or special syringes needed.
Purify intact exosomes (40–200 nm) depending on sample input type.
Purified exosomes are compatible with functional studies, NanoSight® analysis, and electron microscopy.
Exosomal RNA can be extracted using Norgen’s Exosomal RNA Purification technology or other RNA extraction methods.

Product Description

The Urine Exosome Purification Kits provide a fast, reliable, and convenient method to purify and enrich pure intact exosomes from urine samples (250 µL–30 mL).
These kits also allow purification of intact extracellular vesicles (EVs) ready for downstream applications.
The purification is based on Norgen’s proprietary resin and does not require special instrumentation or precipitation reagents.
Purified exosomes are free from protein-bound cell-free RNA, ensuring high-quality exosomal RNA for gene expression studies.

NanoSight® Analysis

Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® to assess exosome size range and concentration.

(이미지에는 텍스트가 포함되어 있지 않으며, 어두운 배경 위에 빛의 점 형태로 엑소좀 입자가 시각화되어 있음.)

Exosomal RNA Analysis

Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit for gene expression analysis (RT-qPCR, microarray, NGS) and biomarker discovery.

Supporting Data

Figure 1. Intact exosomes purified from 5 mL using different purification methods.
Exosomes were purified from 5 mL urine using Norgen’s Urine Exosome Purification Midi Kit (Cat# 57800), Competitor S’s kit, and Competitor L’s kit.
Norgen’s kit isolated 115 nm exosomes with a recovery of 8.36 × 10⁹ particles/mL without impurities.
Broader exosome size range (75–250 nm) and higher concentration compared to competitors.

Figure 2. Intact exosomes purified from 10 mL urine using Norgen’s Midi Kit and Ultracentrifugation.
Exosomes purified using Norgen’s kit (65–195 nm, 7.63 × 10⁸ particles/mL) were smaller and purer than those from ultracentrifugation (125–235 nm, 1.56 × 10⁸ particles/mL).

Figure 2 Image OCR Summary

구분	y축	x축	주요 피크 값 (nm)
Ultracentrifugation	Concentration (×10⁸/mL)	Particle Size (nm)	125, 145, 165, 235
Norgen	Concentration (×10⁸/mL)	Particle Size (nm)	95

Kit Specifications

항목	내용
Urine Input (Cat. 57700)	250 μL – 1 mL
Urine Input (Cat. 57800)	2 mL – 10 mL
Urine Input (Cat. 57900)	11 mL – 30 mL
Size of Exosomes Purified	40 nm – 150 nm
Elution Volume	Variable depending on urine input
Time to Complete 10 Purifications	15 – 30 minutes

Storage Conditions and Product Stability

All buffers should be tightly sealed and stored at room temperature.
Kits are stable for 2 years after shipment.

Important Note

Urine samples stored at -70°C, -20°C, or 4°C may develop precipitates due to protein aggregation.
Removing these precipitates by centrifugation or filtration may cause exosome loss and affect nucleic acid quality.
Use Norgen’s Urine Preservative to preserve nucleic acids and proteins in fresh urine samples at ambient temperature for over 2 years.

Kit Components

Component	Cat. 57700 (50 preps)	Cat. 57800 (25 preps)	Cat. 57900 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	30 mL	50 mL
ExoR Buffer	12 mL	12 mL	12 mL
Mini Filter Spin Columns (2 mL tubes)	50	25	15
Product Insert	1	1	1

Documentation

Protocols

Safety Data Sheets

Flyers

제품 이미지

Exosomes - Nanosight
Figure 1. Intact exosomes purified from 5 mL using different purification methods.
Figure 2. Intact Exosomes were purified from 10 mL urine using Norgen’s Urine Exosome Purification Midi Kit and Ultracentrifugation.