Thermo Fisher Scientific Nkx2.6 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Immunogen sequence:
  LLSPVTSTPF SVKDILRLER ERSCPAASPH PRVRKSPENF QYLRMDAEPR GSEVHNAGGG GGDRKLDGSE
• Antigen sequence identity:
  Mouse 32%; Rat 32%

Target Information

Members of the NK-2 family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart, and pancreas.
Nkx-2.5, also designated cardiac-specific homeobox protein (Csx), is a homolog of the Drosophila tinman protein and is essential for normal cardiovascular development. Expression of Nkx-2.5 during cardiomyogenesis is required for cardiac septation, in which a single atrium and ventricle are separated into four chambers.

Nkx-2.5 binds to DNA as a monomer, a homodimer, or as a heterodimer with Nkx-2.3 or Nkx-2.6, suggesting that specific protein-protein interactions are involved in its transcriptional regulatory function.

Nkx-2.6, also a homolog of the Drosophila tinman protein, is expressed in the caudal pharyngeal pouches, the caudal heart progenitors, the sinus venosus, the outflow tract of the heart, and in a short segment of the gut between stages E8.5 and E10.5 of embryogenesis. Expression of Nkx-2.6 overlaps with that of Nkx-2.5 in the pharynx and heart. However, Nkx-2.6 mutant mice are viable and fertile, suggesting that Nkx-2.6 plays a compensatory function to Nkx-2.5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.