Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Brilliant Violet 605, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–737)
• Brilliant Violet series (480, 650, 711)
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series (436–780)
• Custom conjugation available

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells throughout development except in terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with BCR to induce tyrosine phosphorylation, calcium flux, and B cell proliferation.
SJ25C1 and HIB19 antibodies recognize overlapping epitopes.

Applications Reported:

• Suitable for flow cytometric analysis.

Applications Tested:

• Pre-diluted and validated for flow cytometry using normal human peripheral blood cells.
• Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:

• Brilliant Violet™ 605 (BV605) emits at 605 nm and is excited by a 405 nm violet laser.
• Ensure cytometer compatibility with this fluorochrome.

Staining Panel Recommendations:

• When using multiple Brilliant Violet™, Brilliant Ultra Violet™, or polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Handling Notes:

• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).
  Samples may be stored up to 3 days at 4°C in the dark.
• Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and on certain myeloid leukemia cells. CD19 serves as a signal-amplifying coreceptor for the B cell receptor (BCR), lowering the threshold for antigen receptor-dependent stimulation.
Mutations in CD19 cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
CD19 is a key regulator of normal and malignant B cell proliferation and differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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