Thermo Fisher Scientific ATG4B Recombinant Rabbit Monoclonal Antibody (JE59-97)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JE59-97

Immunogen

Recombinant protein within Human ATG4B aa 221-320/393. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATG4B,uniProtId:Q9Y4P1-1,ncbiNodeId:9606,antigenRange:221-320,antigenLength:393,antigenImageFileName:MA5-50822_ATG4B_Q9Y4P1-1_Rabbit.svg,antigenImageFileNamePDP:MA5-50822_ATG4B_Q9Y4P1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3093861

Product Specific Information

Positive control: 293T cell lysate, Jurkat cell lysate, PC-12 cell lysate, mouse heart tissue lysate, mouse pancreas tissue lysate.

Predicted band size: 44 kDa

Subcellular Location: Cytoplasm, cytosol, Cytoplasmic vesicle, autophagosome, Endoplasmic reticulum, Mitochondrion.

Target Information

Cysteine protease ATG4b is an enzyme which is coded by ATG4b gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4b is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4b, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4b is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.