Thermo Fisher Scientific PKM2 Polyclonal Antibody
PKM2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
| Flow Cytometry (Flow) | 5 µg/mL | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to the internal region of human PKM2 protein (within residues 350–450). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 30% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_11151984 |
Product Specific Information
The target sequence has 95% sequence homology with bovine.
Target Information
PKM2 (Pyruvate Kinase M2) catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. It interacts with thyroid hormone, mediating metabolic effects induced by thyroid hormones. PKM2 also binds to Opa protein, a bacterial outer membrane protein involved in gonococcal adherence and invasion of human cells, indicating a role in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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