Thermo Fisher Scientific HIF1A Polyclonal Antibody
HIF1A 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot에 적합합니다. 인간에 반응하며, 프라이메이트·쥐·생쥐에도 예측 반응합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS/30% 글리세롤 버퍼에 보관됩니다. 연구용 전용 제품입니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide made to an internal portion of the human protein (within residues 700–750). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 30% glycerol |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153628 |
Product Specific Information
This antibody is predicted to react with primate, rat, and mouse based on 100% sequence homology; 93% sequence homology with chicken and bovine.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer composed of alpha and beta subunits, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors.
HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia, activating transcription of over 40 genes including erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. It interacts with p53 and p300/CBP complexes to modulate hypoxia-responsive gene expression.
Hypoxia contributes significantly to diseases such as myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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