Thermo Fisher Scientific LDHA Polyclonal Antibody
LDHA 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 다양한 종(Bovine, Human, Mouse, Pig)에 반응하며 WB, IHC, ICC 등 여러 응용에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태로, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | View publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 1:200 | - |
| Flow Cytometry (Flow) | Assay-Dependent | - |
| ELISA | Assay-Dependent | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Pig |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide made to a C-terminal portion of the human LDHA protein (within residues 280–332) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152155 |
Product Specific Information
This antibody is predicted to react with bovine and porcine based on 100% sequence homology.
Target Information
Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
The three human LDHs have 84–89% sequence similarities and 69–75% amino acid identities.
There are five different LDH isoenzymes based on the proportion of M and H chains in the tetrameric structure.
LDHA is hypoxia-inducible and its expression is controlled by the transcriptional activity of HIF1α. LDHB is upregulated in many cancers. Mutations in LDHA can lead to Glycogen Storage Disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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