Thermo Fisher Scientific TRPC6 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 10–50 µg/mL

Immunohistochemistry (IHC)

• Tested Dilution: 10–50 µg/mL

Product Specifications

Product Specific Information

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: TRPC6.

Target Information

Trpc6 forms a receptor-activated calcium channel in the cell membrane. The Trpc6 channel is activated by diacylglycerol (DAG) and is controlled by a phosphatidylinositol second messenger system. Activation occurs independently of protein kinase C and is not triggered by low intracellular calcium levels.

Defects in the Trpc6 gene cause focal segmental glomerulosclerosis 2 (FSGS2). Trpc6 belongs to the mammalian transient receptor potential (TRP) superfamily, specifically the “canonical TRP” (TRPC) family. Members of this family are components of receptor-regulated cation channels and are activated through PLC-coupled receptors.

The TRPC6/6/7 subfamily can be activated by DAG analogs, suggesting a mechanism of activation by PLC-coupled receptors. When expressed in transfected cells, Trpc6 acts as a non-selective, store-independent, receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. Evidence indicates that Trpc6 encodes endogenous DAG-activated receptor-operated cation channels in vivo.

Diseases associated with TRPC6 include:

• Glomerulosclerosis, Focal Segmental, 2
• Focal Segmental Glomerulosclerosis

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.