Thermo Fisher Scientific LDHA Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.3–1 µg/mL

Product Specifications

Product Specific Information

• This antibody is expected to recognize isoform 1, 2, and 3 (NP_005557.1; NP_001128711.1; NP_001158886.1).
• Tested in Peptide ELISA: antibody detection limit dilution 1:64,000.

Target Information

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+.
In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form).
The three human LDHs have 84–89% sequence similarities and 69–75% amino acid identities.
There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure.
LDHA is hypoxia-inducible and its expression is directly controlled by the transcriptional activity of hypoxia-inducible factor 1a (HIF1a).
LDHB is also known to be upregulated in many cancers.
Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.