Thermo Fisher Scientific ATG12 Monoclonal Antibody (GT166)
ATG12 단백질을 인식하는 Mouse monoclonal antibody (Clone GT166). Western blot, ICC/IF, PLA 등 다양한 응용에 적합. Human 및 Mouse 반응성 확인. Protein G 정제, 무보존액 PBS 포맷으로 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| in situ PLA (PLA) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT166 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG12 (proprietary sequence) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735122 |
Product Specific Information
- Positive Control: 293T, A431, HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12
- Predicted Reactivity: Pig (81%), Chimpanzee (96%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 interacts with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. It helps preserve cellular nutrients under starvation conditions and regulates turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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