Thermo Fisher Scientific H3K4me2 Polyclonal Antibody
H3K4me2에 특이적인 Rabbit Polyclonal Antibody로 인간 시료에 반응합니다. Western blot, IHC, ICC/IF, IP, ChIP 등 다양한 응용에 적합하며 항원 친화 크로마토그래피로 정제되었습니다. 안정적인 PBS 버퍼에 보존되어 연구용으로 사용됩니다.
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Thermo Fisher Scientific H3K4me2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:500–1:1,000 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 2–16 of Human Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.52 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549385 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes, including methylation, acetylation, and phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
- PA5-31912_H3K4me2_P68431-1_Rabbit.svg
- PA5-31912_H3K4me2_P68431-1_Rabbit_PDP.jpeg
- PA5-31912_H3K4me2_Q71DI3-1_Rabbit.svg
- PA5-31912_H3K4me2_Q71DI3-1_Rabbit_PDP.jpeg
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