Thermo Fisher Scientific NFYB Polyclonal Antibody
NFYB 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot, IHC, ICC/IF, IP, ChIP 등에 사용 가능. 인간, 마우스, 랫트에 반응하며 항원 친화 크로마토그래피로 정제됨. PBS 기반 용액으로 -20°C 장기 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
| ChIP assay (ChIP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 33–207 of Human NFYB |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.42 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549386 |
Product Specific Information
- Recommended positive controls: HeLa, HeLa nuclear extract, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2
- Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (92%), Pig (99%), Rhesus Monkey (100%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is composed of three subunits: NF-YA, NF-YB, and NF-YC, all required for DNA binding.
Each subunit contains conserved segments homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of NF-YB and NF-YC are suggested to interact via a histone-fold “handshake” motif, similar to H2B and H2A proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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