Thermo Fisher Scientific H3K9me3 Polyclonal Antibody
H3K9me3 타깃의 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF, IP, ChIP 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피 정제. 장기 보관 시 -20°C 권장.
- 카탈로그번호
- PA531910
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:1,000 |
| ChIP assay (ChIP) | Assay-dependent |
| Peptide Array (Array) | 0.25 µg/mL |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 6–20 of Human Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549383 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is a DNA-binding protein found in chromatin of all eukaryotic cells. Together with other core histones, it forms the nucleosome structure. Histones play key roles in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histone H3, such as methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression by altering accessibility or recruiting transcription factors. The N-terminal tail of histone H3 is particularly subject to such modifications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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