Thermo Fisher Scientific FAK Polyclonal Antibody
FAK 단백질을 검출하기 위한 Thermo Fisher Scientific의 폴리클로날 항체. 다양한 종에 반응하며 IHC(P) 등 연구용으로 적합. 항원 친화 크로마토그래피로 정제된 고순도 항체로, 세포 부착 및 신호전달 연구에 활용 가능.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific FAK Polyclonal Antibody
Thermo Fisher Scientific FAK Polyclonal Antibody
Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 13 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bat, Bovine, Dog, Chicken, Hamster, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 16 amino acid peptide from kinase domain of human FAK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550464 |
Product Specific Information
Percent identity with other species by BLAST analysis:
Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Turkey, Chicken, Lizard (100%); Rabbit, Xenopus, Zebrafish (94%); Platypus, Pufferfish (88%).
For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling.
FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle.
FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors.
Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase.
In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin.
In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site-directed mutagenesis.
Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.