Thermo Fisher Scientific CD81 Monoclonal Antibody (1D6), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

-

Product Specifications

Species Reactivity

Sheep, Chimpanzee, Goat, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D6

Immunogen

OCI-LY8 cells aggregated by another CD81 antibody (clone 5A6). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD81,uniProtId:P60033-1,ncbiNodeId:9606,antigenRange:1-236,antigenLength:236,antigenImageFileName:MA1-81261_CD81_P60033-1_House_mouse.svg,antigenImageFileNamePDP:MA1-81261_CD81_P60033-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_929487

Product Specific Information

Clone 1D6 is a potent CD81 reagent that induces homotypic adhesion and has powerful anti-proliferative effects.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD81 antibody, clone 1D6 recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family.

Target Information

CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells. In muscles, CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus. Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that is known to complex with integrins. CD81 appears to promote muscle cell fusion and support myotube maintenance. CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas. CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.