Thermo Fisher Scientific SF1 Polyclonal Antibody
Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. Human, Mouse, Rat 반응성. Rabbit Polyclonal Antibody. 항원 친화 크로마토그래피로 정제. -20°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 0.5–1 µg/mL |
| Immunohistochemistry (Frozen) (IHC-F) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human splicing factor 1 (11–30aa DFPSKKRKRSRWNQDTMEQK) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide, 0.05 mg thimerosal |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2747099 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Splicing factor 1 is a nuclear pre-mRNA splicing factor that recognizes the intron branch point sequence at the 3′ splice site together with the large subunit of U2 auxiliary factor (U2AF). It is required for early stages of spliceosome assembly and plays roles in nuclear pre-mRNA retention and transcriptional repression. The protein contains:
- An N-terminal U2AF ligand motif
- A central hnRNP K homology motif and quaking 2 region
- A zinc knuckles domain
- A C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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