Thermo Fisher Scientific JNK3 Monoclonal Antibody (2B10H12)
인간 및 랫드 시료에 반응하는 JNK3 단클론 항체로 Western blot 및 ELISA에 적합합니다. 정제된 인간 MAPK10 재조합 단백질을 면역원으로 제작되었으며, 단백질 G로 정제된 액상 형태입니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B10H12 |
| Immunogen | Purified recombinant fragment of human MAPK10 (AA: 180–329) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787418 |
Product Specific Information
MA5-31795 has been tested in indirect ELISA.
Target Information
MAPK10 (JNK3) is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). It is involved in neuronal apoptosis and phosphorylates MAP2K4. MAP kinases act as integration points for multiple biochemical signals and participate in processes such as proliferation, differentiation, transcription regulation, and development.
JNK3 plays regulatory roles in signaling pathways during neuronal apoptosis through phosphorylation and nuclear localization. Beta-arrestin 2 interacts with and stimulates phosphorylation of JNK3 by MAP kinase kinase 4 (NhpK4). Cyclin-dependent kinase 5 can phosphorylate and inhibit JNK3 activity, which may help prevent neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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