Thermo Fisher Scientific MIB1 Monoclonal Antibody (2A7B1)
MIB1 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, Flow cytometry, ELISA에 사용 가능. Human 및 비인간 영장류 반응성. Protein G 정제, 1 mg/mL 농도, PBS buffer에 0.05% sodium azide 포함. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2A7B1 |
| Immunogen | Purified recombinant fragment of human MIB1 (AA: 6–221) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787419 |
Product Specific Information
MA5-31796 has been tested in indirect ELISA.
Target Information
MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB may also mediate ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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