Thermo Fisher Scientific Sendai virus HN Monoclonal Antibody (1A6), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1.0 µg/test

-

Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1A6

Immunogen

Sendai Virus

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Alexa Fluor 647
• Biotin
• PE
• PE-Cyanine7
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2848440

Product Specific Information

Description: This 1A6 monoclonal antibody recognizes hemagglutinin-neuraminidase of Sendai virus. This clone has been successfully used in flow cytometry, immunocytochemistry and has been reported for neutralization. The CTS CytoTune-iPS 2.1 Sendai Reprogramming Kit and the CytoTune-iPS 2.0 Sendai Reprograming Kit utilize Sendai virus particles to deliver Yamanaka factors into target cells in order to reprogram them into induced Pluripotent Stem Cells (iPSC). Clone 1A6 can be used to detect the presence of the residual Sendai virus after transduction and subsequent tissue culture.

Applications Reported: This 1A6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1A6 antibody has been tested by flow cytometric analysis of Sendai virus-infected cells. This may be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Target Information

Sendai virus is a single stranded RNA respirovirus from the family Paramyxoviridae, which replicates in the respiratory tract and infects certain mammals, including mice, hamsters, rats and guinea pigs. The virus is highly contagious and in the wild is transmitted through contact with aerosolized respiratory secretions. The Hemagglutinin-Neuraminidase (HN) protein of the Sendai virus facilitates cell entry and infection by binding and cleaving terminal sialic acids present on many cell surface glycoproteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.