
Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합형 Rabbit anti-Goat IgG (H+L) 교차흡착 2차 항체로, Western blot, IHC, ELISA에 적합합니다. 높은 특이성과 감도를 제공하며, 인간·마우스·랫 IgG에 대한 교차 반응이 최소화되었습니다. 친화 크로마토그래피로 정제된 고순도 제품입니다.
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Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534813 |
| Target | IgG |
| Cross Adsorption | Against human, mouse and rat IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- No reactivity observed to non-immunoglobulin goat serum immunoglobulins, or IgG from human, mouse, or rat.
Target Information
Anti-Goat secondary antibodies are affinity-purified with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of goat IgG targets.
Secondary antibodies provide versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind a single primary antibody.
Typically generated by immunizing the host with pooled immunoglobulins from the target species, then purified and conjugated (e.g., HRP, AP, fluorescence) for high specificity.
Available Conjugate Formats
- Alexa Fluor 488, 546, 555, 568, 594, 633, 647, 680
- AP
- Biotin, Biotin-XX
- DyLight 488, 550, 594, 650, 680, 800
- FITC, TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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