Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, AP
Rabbit anti-Goat IgG (H+L) AP-conjugated secondary antibody로 Western blot, IHC, ELISA에 사용 가능. 인간, 마우스, 랫트 IgG에 교차흡착 처리되어 높은 특이성과 낮은 배경 신호 제공. 액상 형태로 2.5 mg/mL 농도, 장기 저장 시 -20°C 보관 권장.
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Thermo Fisher Scientific Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, AP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30mM triethanolamine, 1% BSA, 5mM MgCl₂, 0.1mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534816 |
| Target | IgG |
| Cross Adsorption | Against human, mouse and rat IgG |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor 680
- Biotin
- Biotin-XX
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 680
- DyLight 800
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are useful in detection, sorting, or purification of the specified target. Secondary antibodies offer increased versatility, enabling use with various detection systems such as HRP, AP, and fluorescence. They also provide enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Most secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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