Thermo Fisher Scientific ELL3 Polyclonal Antibody
Rabbit polyclonal antibody against human ELL3, validated for IHC(P) and ICC/IF. Recombinant protein immunogen, affinity purified. Supplied in PBS with 40% glycerol. Suitable for enhancer-binding elongation factor studies in stem cell differentiation re...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ELL3. Recombinant protein control fragment (Product # RP-89344) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640990 |
Product Specific Information
Immunogen sequence:
HRGYLRLPGP GWSCLFSFIV SQCCQEGAGG SLDLVCQRFL RSGPNSLHCL GSLRERLIIW AAMDSIPAPS SVQGHNLTED ARHPESWQNT GGYSEGDAVS QPQMALEEVS VSDPLASN
Highest antigen sequence identity to orthologs:
- Mouse: 70%
- Rat: 71%
Target Information
Enhancer-binding elongation factor that specifically binds enhancers in embryonic stem cells (ES cells), marking them for future activation during stem cell specification. It binds enhancer regions of both active and poised genes and is required for proper RNA polymerase II occupancy at developmentally regulated genes in a cohesin-dependent manner.
Likely primes developmentally regulated genes for later recruitment of the super elongation complex (SEC) during differentiation and is required for P-TEFb recruitment within SEC. May be preloaded on germ cell chromatin, suggesting a role in early enhancer marking.
Functions as an elongation factor component of the SEC, enhancing RNA polymerase II transcription elongation, and as part of the little elongation complex (LEC) regulating small nuclear RNA (snRNA) gene transcription (PubMed:22195968).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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