Thermo Fisher Scientific PDHA1 Recombinant Rabbit Monoclonal Antibody (JF996-0)
PDHA1 단백질을 인식하는 재조합 토끼 단일클론 항체로 높은 특이성과 재현성을 제공합니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. HEK293 세포에서 발현되었으며 Protein A로 정제된 액상 형태입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF996-0 |
| Immunogen | Synthetic peptide within Human PDHA1 aa 100–145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809822 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO₂, linking glycolysis to the tricarboxylic acid (TCA) cycle.
The PDH complex includes:
- Pyruvate dehydrogenase (E1)
- Dihydrolipoamide acetyltransferase (E2)
- Lipoamide dehydrogenase (E3)
The E1 enzyme is a heterotetramer composed of two alpha and two beta subunits. The PDHA1 gene encodes the E1 alpha 1 subunit containing the active site, essential for PDH function. Mutations are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
Alternative splicing generates different isoforms of this gene.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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