Thermo Fisher Scientific TNFA Monoclonal Antibody (OTI4B9), TrueMAB
인간 TNFα를 인식하는 mouse monoclonal antibody로 Western blot 및 유세포분석에 사용됩니다. TrueMAB 형식으로 고순도 정제되어 있으며, 동결건조 상태로 제공됩니다. 재구성 후 약 1 mg/mL 농도로 사용 가능하며, 연구용으로 적합합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Flow Cytometry (Flow)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4B9 |
| Immunogen | Full length human recombinant TNF protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is primarily secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cell activity, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It exists as multimers but appears as a single 17 kDa band under non-reducing SDS-PAGE conditions. TNF alpha plays roles in immune responses to infections, tumor necrosis, and has been implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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