Thermo Fisher Scientific Phospho-AMPK alpha 2 (Ser173) Polyclonal Antibody
Phospho-AMPK alpha 2 (Ser173) 폴리클로날 항체로 인간, 마우스, 랫트에 반응. Western blot, IHC, ICC/IF, Flow, ELISA 등 다양한 응용 가능. Protein A로 정제된 고순도 액상 항체로 에너지 대사 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human PRKAA2 around the phosphorylation site of Ser173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits. It serves as an important energy-sensing enzyme that monitors cellular energy status. Upon metabolic stress, AMPK becomes activated and phosphorylates acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), key enzymes regulating fatty acid and cholesterol biosynthesis. Studies in mice suggest that this catalytic subunit may influence insulin sensitivity and maintain myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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