Thermo Fisher Scientific LRRC8A (extracellular) Polyclonal Antibody
LRRC8A 단백질의 세포외 영역을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC(F), Flow cytometry에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 완충액에 보관. 연구용으로만 사용.
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Thermo Fisher Scientific LRRC8A (extracellular) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 |
| Flow Cytometry (Flow) | 5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)DTGPTGIKYDLDRH, corresponding to amino acid residues 91–104 of human LRRC8A, 1st extracellular loop |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution: 25 µL, 50 µL, or 0.2 mL double distilled water (DDW), depending on sample size.
The antibody ships as a lyophilized powder at room temperature. Upon arrival, store at -20°C.
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, store small aliquots at -20°C.
Avoid multiple freezing and thawing cycles. Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
LRRC8A is an essential component of the volume-regulated anion channel (VRAC, also known as VSOAC channel), which maintains constant cell volume in response to osmotic changes.
The VRAC channel conducts iodide better than chloride and may also conduct organic osmolytes like taurine.
LRRC8A is involved in B-cell development (pro-B to pre-B transition) and T-cell development.
It is unclear whether LRRC8A forms the pore or associates with it to mediate ion selectivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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