Thermo Fisher Scientific Human IL-1 beta Recombinant Protein
재조합 인간 IL-1β 단백질로 면역학적 분석의 양성 대조군으로 사용 가능. E. coli 발현 시스템에서 생산된 His-tag 단백질로 순도 95% 이상. 염증 반응 연구 및 사이토카인 신호 전달 연구에 적합. -20°C에서 보관, 동결-해동 반복 피할 것.
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Applications
- Western Blot (WB): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Ala117–Ser269 (N-terminal His-tag 포함) |
| Tag | His-tag |
| Class | Recombinant |
| Type | Protein |
| Purity | >95%, SDS-PAGE (reducing conditions) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.19 mg/mL |
| Purification | Purified |
| Storage Buffer | 20 mM Tris-HCl (pH 8), 250 mM NaCl, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
RP-75585 contains purified recombinant human IL-1 beta protein with an N-terminal hexahistidine tag.
RP-75585 may be used as a positive control in many immunological assays.
Target Information
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized as a 31 kDa inactive pro-form that accumulates in the cytosol and is cleaved into an active 17 kDa form upon inflammasome activation.
IL-1β lacks a classical signal peptide and is secreted via an alternate pathway alongside caspase-1. It signals through IL-1RI and IL-1RII receptors, shared with IL-1α, and its activity is regulated by IL-1 Receptor Antagonist (IL-1RA).
IL-1β plays a key role in innate immune defense by inducing other proinflammatory cytokines and initiating acute-phase responses. Elevated IL-1β levels are associated with chronic inflammatory conditions, and IL-1β neutralizing antibodies have potential therapeutic value.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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