Thermo Fisher Scientific ARAF Monoclonal Antibody (OTI2F5), TrueMAB
인간 ARAF 단백질(aa 145-365)에 특이적인 Mouse IgG1 단일클론 항체. Western blot에 최적화되어 있으며, 동결건조 형태로 제공. PBS(8% trehalose) 버퍼에 보존되고, 재구성 후 1 mg/mL 농도로 사용 가능. 연구용으로만 사용.
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Applications
- Western Blot (WB): Tested dilution 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 145–365 of human ARAF produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Several serine/threonine protein kinases act as intermediates in signal transduction pathways, including ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1.
Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is the cellular homolog of v-Raf, the product of the transforming gene of murine sarcoma virus.
A-Raf, another member of the Raf gene family, shows high homology with Raf-1 within the kinase domain and is mainly expressed in urogenital tissues and kidney, with low expression in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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